New enzymatic assay using phospholipase D to measure total calcium in serum.

1. Pearson TC, Messinezy M, Westwood N, Green AR, Bench AJ, Huntly BJ, et al. Polycythemia vera updated: diagnosis, pathobiology, and treatment. Hematology (Am Soc Hematol Educ Program) 2000:51–68. 2. Tefferi A. Polycythemia vera: a comprehensive review and clinical recommendations. Mayo Clin Proc 2003;78:174–94. 3. Birgegard G, Wide L. Serum erythropoietin in the diagnosis of polycythaemia and after phlebotomy treatment. Br J Haematol 1992;81:603–6. 4. Remacha AF, Montserrat I, Santamaria A, Oliver A, Barcelo MJ, Parellada M. Serum erythropoietin in the diagnosis of polycythemia vera. A follow-up study. Haematologica 1997;82:406–10. 5. Messinezy M, Westwood NB, El-Hemaidi I, Mardsen JT, Sherwood RS, Pearson TC. Serum erythropoietin values in erythrocytoses and in primary thrombocythaemia. Br J Haematol 2002;117:47–53. 6. Casadevall N. Determination of serum erythropoietin. Its value in the differential diagnosis of polycythemias. Nouv Rev Fr Hematol 1994;36: 173–6. 7. Lindstedt G, Lundberg PA. Are current methods of measurement of erythropoietin (EPO) in human plasma or serum adequate for the diagnosis of polycythaemia vera and the assessment of EPO deficiency. Scand J Clin Lab Invest 1998;58:441–58. 8. Spivak JL. Polycythemia vera: myths, mechanisms, and management. Blood 2002;100:4272–90. 9. Pearson TC. Evaluation of diagnostic criteria in polycythemia vera. Semin Hematol 2001;38:21–4. 10. Jaffe ES, Lee Harris N, Stein H, Vardinan JW, eds. World Health Organization classification of tumours. Pathology and genetics of tumours of haematopoietic and lymphoid tissues. Lyon, France: IARC Press, 2001:32–4. 11. Mossuz P, Girodon F, Donnard M, Latger-Cannard V, Dobo I, Boiret N, et al. Diagnostic value of serum erythropoietin level in patients with absolute erythrocytosis. Haematologica 2004;89:1194–8. 12. Benson EW, Hardy R, Chaffin C, Robinson CA, Konrad RJ. New automated chemiluminescent assay for erythropoietin. J Clin Lab Anal 2000;14:271–3. 13. Owen WE, Roberts WL. Performance characteristics of the IMMULITE 2000 erythropoietin assay. Clin Chim Acta 2004;340:213–7. 14. Andreasson B, Carneskog J, Lindstedt G, Lundberg PA, Swolin B, Wadenvik H, et al. Plasma erythropoietin concentrations in polycythaemia vera with special reference to myelosuppressive therapy. Leuk Lymphoma 2000;37: 189–95.